Реферат: Quantification of osteocyte lacunar density and anisotropy of vascular canals in compact bone

-Introduction, bone structure, remodelation, osteoporosis,

Remodelling of Haversian bone may result in both spatial and temporal variability of osteocyte lacunar density and arrangement of vascular canals.

-Parameters and methods in bone histomorphometry, tetracycline labeling, disector

The most commonly used histomorphometric parameters: [1]

1- Structural parameters

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Measures that describe the biopsy specimen

-Core width (mm)

C.Wi Overall size of the biopsy specimen, refers to the width of the entire biopsy specimen or core. It is the distance between the inner and outer periosteal surfaces

-Cortical width (μm)

Ct.Wi Distance between periosteal and endocortical surfaces, he sum of the widths of inner and outer cortices of the biopsy specimen.

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-Bone volume / tissue volume (%)

BV/TVSpace taken up by mineralized and unmineralized bone relative to the total size of a bone compartmen, is cancellous bone volume as a percentage of tissue volume. The numerator includes matrix, whether mineralized or not, and the denominator includes cancellous bone, marrow, and associated soft tissue.

Measures that describe the configuration of trabeculae in space:

Trabecular thickness (μm)

Tb.Th Is the mean distance across individual trabeculae

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Trabecular number (/mm)

Tb.N Number of trabeculae that a line through a trabecular compartment would hit per millimeter of its length, (BV/TV)/Tb.Th

- Trabecular separation

Tb.Sp Is the mean distance between individual trabeculae

2- Static formation parameters

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Osteoid thickness (μm)

O.Th Distance between the surface of the osteoid seam and mineralized bone

Osteoid surface / bone surface (%)

OS/BS Percentage of bone surface covered by osteoid

Osteoid volume / bone volume (%)

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OV/BV Percentage of bone volume consisting of unmineralized osteoid

Osteoblast surface / bone surface (%)

Ob.S/BS Percentage of bone surface covered by osteoblasts

Wall thickness (μm)